JR Screen Ruler is a tool that can help you check out sizes of objects and distances between objects on your screen. It defaults to pixels (although it can display other units as well), and can easily be dragged around the screen to position it. A quick click will rotate it from horizontal to vertical orientation, and a slide control lets you tweak the length of your on-screen ruler. You can also play with color, transparency, and more, so that the tool will be most helpful to you.
A free download, JR Screen Ruler is a Windows Application. It should be right at home on any Win32 platform, from Windows 95 on up.